PH Essays & Research Papers

Cesar Bizarroque Wesam Daker Bio 1107k-11 10/25/14 How pH levels impact tyrosinase activity Abstract: The purpose of our research utilizing the different pH levels was to test how a specific pH level would impact tyrosinase activity. First we added 4.0 mL of pH in each corresponding test tube and then added 0.5 mL of substrate (catechol) into each test tube. In the instructions it says to apply your 0.5 mL of tyrosinase (potato extract) as well but you have to blank the spectrophotometer...

Instructions Preparing the Cabbage-Juice Indicator 1 Chop one head of red cabbage leaves into about 1-inch pieces and place them in a blender. Fill the blender about half full of water and blend the mixture at high speed for about 30 seconds. 2 Place a coffee filter inside a strainer and pour the cabbage juice through the coffee filter into a quart-sized jar. Sponsored Links Forensic Science Courses Take Forensic Science Classes Online or Nearby - Start In...

pH of Household Bases and Acids Objective: To determine if common household substance are acids, bases or neutral. Standards: Materials: Litmus paper Lemon juice Bleach (ammonia) Diet Pepsi Mouthwash Hand Soap Isopropyl Alcohol 70% Baking Soda Solution Coffee Apple cider Red Wine Vinegar Tap water Filtered water Safety and Disposal: Material Hazard Disposal Ammonia (bleach) White cloudy solution. Strong ammonia odor. Mildly toxic by ingestion and inhalation. Irritating...

Effects of Temperature and pH on Enzyme Function Chelsie Mesa Section 0479 Robin Cotter Introduction The purpose of this experiment is to identify three unknown enzymes. This is done by using different temperature and pH to affect the function of the enzyme, which ultimately, will affect how much maltose is produced. Enzymes are proteins that help catalyze chemical reactions. In enzymatic reactions, the molecules at the beginning of the process are called substrates, and the...

Acids, Bases, and Buffers Introduction: The pH scale is used to determine how acidic or basic a solution is, ranging from 1-14. The most acidic of all acids are at a pH level of 1 and the most basic of all bases are at 14. The neutral pH level is 7, which is what drinking water is. The pH level is determined by the amount of H+ ions present in a solution, and the more H+ ions there are the more acidic it is, and the lack of these ions results in more basic solutions. One distinguishing...

pH pH means Power of Hydrogen or Potential of Hydrogen. pH is a scale of acidity from 0 to 14. It tells how acidic or alkaline a substance is. More acidic solutions have lower pH. More alkaline solutions have higher pH. Substances which are not acidic or alkaline i.e neutral usually have a pH of around 7. Acids have a pH less than 7. Alkalis have a pH greater than 7. There are a lot of ways for finding the pH of something. One way is to use litmus paper. The pH paper is able to tell how...

MEASURING pH Date of Lab: 02/09/13 Date Report submitted: 02/26/13 Purpose of the experiment By doing this experiment, we were trying to learn how to measure a pH with a pH meter to determine whether a solution is weakly, moderately or strongly acidic or basic; but also to compare the different data founded to see how the addition of certain liquid to a solution may affect the pH of the initial solution. Materials We used: * Tap water, distilled water, Soda and milk as the main...

PH is the measure of acid or alkaline in the blood in the human body. The pH level is measured on a scale from 1 to 14; 1 being the most acidic and 14 being the most alkaline. The normal blood range should be closest to the number 7.4 on the pH scale. Once the body reaches a number lower than 7 it is considered too acidic and no longer optimal and metabolism will become out of balance. Many different diseases and injuries can cause this condition. Different forms of acidosis can occur to the...

The pH Scale is a scale made to show how acidic or alkaline a substance is. The pH of something is measured with pH paper which can vary from wide range paper, which covers all ph’s, but is not as accurate at some others. There are pH papers that get very specific that can have a range such as 2 or 3 and get much more precise. The neutral on the scale is 7. Anything that is below 7 is considered acidic and anything above 7 is considered alkaline. Acidic substances contain more H+ than OH-....

Name: Date: 17 Aug 2014 Instructor’s Name: Assignment: SCI103 Phase 1 Lab Report Title: Measuring pH Levels Instructions: Enter the Virtual Lab, and conduct the experiments provided before going out into the virtual field for additional research. Please type your answers on this form. When your lab report is complete, submit it to the Submitted Assignments area of the Virtual Classroom. Part I: Answer the following questions while in the Phase 1 lab environment. Section...

Lab Report: Purpose: The Objective of this lab was to learn how to measure the pH (or acidity) of commonly known fluids, using the correct tools and procedures. To then use that data to document the changes noticed when mixing those same fluids and changing their respective pH levels. Materials: In order to conduct this experiment several pieces of equipment and other materials were needed. The first item was a graduated cylinder, which was used in order to measure out the...

pH plays a very important role in our lives. It is used in several applications. For example, a swimming pool must be constantly maintained by checking its pH. If the water becomes too acidic or too basic, acidic or basic chemicals must be added to the water to balance out the number of H+ ions and OH- ions. http://en.wikipedia.org/wiki/File:PH_Scale.svg pH also is important when it comes to the body. Whenever someone gets heartburn, stomach acid builds up and causes deep pain near the opening...

PH at the UN Alfredo Vidal Ceballos 7/10/2014 Group Support Part 1: Solution Group Name and M. cons. Chem formula Type Experimental pH 1 Deidre Acetic Acid 0.1M HC2H3O2 Weak Acid 3.03 3.03 2 Acetic Acid 1M HC2H3O2 Weak Acid 2.59 2.54 3 Hydrochloric Acid 0.5M HClStrong Base 0.85 0.90 4 Jonah Nitric Acid 0.1M HNO3 Strong Acid 2.14 2.14 5 Sodium Acetate 1M NaC2H3O2 Weak Base 9.42 9.42 6 Potassium Dihydrogen Phosphate 0.1M KH2PO4 Weak Acid 5.47 5.47 7 Andy Potassium Hydrogen Phosphate...

 The Effects of pH on Slime Mold Dg Nicole Harris BIOL181 – 059 1 December 2011 The theory that slime mold is attracted to acidic solutions was examined by the slime mold, Dg. Dg’s preference of pH was examined by using a variety of pH solutions in a petri dish. The data suggests that the growth of fruiting bodies observed in pH 9 was comparable to that of pH 4. INTRODUCTION The 2002 scientific...

 The Spetrophotometric Determination of pH Instrumentation Lab Introduction This experiment is designed to find the pH of an unknown buffer. We find this by adding acid/base indicator of known Ka then by using a spectrophotometric measurement device to measure the absorbance of the solution. Because of an overlap difference between the spectra for acid and base forms, the molar absorptivity for each form will be measured at...

Lab II: Acids or Bases? 1. Acids Bases Sprite Milk Orange juice Windex Vinegar Dish detergent. 2. There are multiple pros and cons for using different types of indicators to determine the Ph level of a substance. When using a paper indicator you can easily get the color of the Ph strip mixed up with another color which will affect the results of the Ph level. Also, a Ph indicator will give you one solid cold, so will a...

 The effect on the pH of Distilled water, Potato Solution and Commercial Buffer, when Hydrochloric acid (0.1 mol/L) and Sodium Hydroxide (0.1 mol/L) is added Mahima Mandava Mrs. Haist September 23rd, 2014 SBI4U1 Background Information: The pH is the measurement of how acidic or how basic a substance can be. The pH scale is used to measure how acidic or basic a living cell can be. The pH scale ranges from 0-14; 0-6 being acidic, 8-14 being basic and 7 being neutral. There are many...

Abstract Title of this experiment is the acid-base indicator from natural dyes. This experiment is to investigate the colour variation of solution extracted from pigment of vegetable and fruit skin according to pH value and to deduce the ability of the extraction to act as an indicator in titration. The problem statement of this experiment is how does the colour of solution extracted from pigment of vegetable and fruit varies according to pH value of universal indicator and can...

------------------------------------------------- Date Due: July 25, 2013 ------------------------------------------------- Date Submitted: July 25, 2013 ------------------------------------------------- ------------------------------------------------- ------------------------------------------------- ------------------------------------------------- ------------------------------------------------- ------------------------------------------------- EXPERIMENT...

Michael Pitner Chem 253- TA: Gia Hoang Lab 1: Effect of pH on a Food Preservative 9-1-2011 Purpose The purpose of this lab was to determine if a new substance forms when sodium benzoate is placed in a simulated stomach acid. Theory Benzoic acid can be found in a wide variety of plants. It is used as a natural preservative in many foods. Sodium benzoate, the salt of benzoic acid, has become a more popular choice as a food preservative because it is more soluble in water than benzoic...

Objective: To investigate how pH affects the rate of protein digestion Hypothesis: I predict that since pepsin breaks down the material the quickest at a pH of 2 in the stomach, test tubes #3 will reduce the greatest because it has pepsin to further break down the egg. Whereas the other test tubes will have a smaller reduction. I think test tube #2 will have the second largest reduction rate and then #6 because they all will have a greater rate at breaking down the material, Then, I predict the...

 In a world of such various differences, human beings since the beginning of time have wanted to find ways to discover what makes said differences, different. Hot vs. cold, soft vs. hard, acid vs. base. For each of the named, we have methods of gauging the difference between one another, and the severity of said difference. For heat we have temperature, for hardness we have scales such as the Rockwell scale, and for acidity or basicness, we have the pH scale. Common examples of acids include...

The effects of pH on mung beans Gloria Cheng Winsy Cheung Lily Wong Christine Yen January 15, 1998 Abstract This experiment explores how different pH environments affect the growth of mung beans. The mung beans were grown in water with various pH levels, consisted of pH levels 5, 6, 7, 8, and 9. A replicated design was used consisting of 3 runs for each pH level. The following results are listed in order of pH levels allowing most growth to least growth of the mung beans: 7, 8, 6,...

Florencia Rodriguez October 20, 2010 IB Biology SLII The effect of three different pH levels (4, 7, 10) on the rate of germination of spanish beans Research Question: What is the effect of three different pH levels (4, 7, 10) on the rate of germination of spanish beans? Independent Variable: The different pH levels in the water would be the independent variable in this lab. Three different pH levels were tested, 4, 7 and 10, both 4 and 10 were chosen because they are 3 pH levels away...

 pH and Living Systems I. Purpose: To observe the effects of pH change on an organic molecule. II. Materials: pH paper Droppers Ammonia Beakers (50ml) Paper towels water Glass stirring rods lemon juice forceps III. Procedure: Part 1: Initial pH testing 1) First use the wide range pH paper to test the pH of the liquids given. 2) When you test with the wide range paper first (which reads pH from 0-13) be...

Experiment #6: Colorimetric Determination of pH Almira, Faerie Carleen Lucile L. Gallardo, Charlotte O. Group #6, Chemistry 18.1, MHEG1, Ma’am Arlou Angeles September 23, 2013 I. Abstract The acidity of the four unknown solutions were determined with the use of colorimetry using McIlvaine’s buffer solutions varying in proportion of its constituents (disodium phosphate and citric acid). These buffer solutions were subjected to the addition of corresponding pH indicators and the variation...

Title: pH and buffer solutions Aim This experiment was carried out to determine the role of buffer solution and the factor which affect the buffer capacity. Besides, this experiment was carried out to investigate the solubility of protein casein over a range of pH concentration. This experiment also was carried out to determine the isoelectric point of the casein and the effect of the isoelectric point toward the casein solution. Methods Verification of the Henderson-Hasselbalch equation...

Biology Lab Report Research Question: Effects of pH on amylase activity Introduction: Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case, amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example, may taste sweet to us, because they contain starch. The optimum pH for...

Lab on pH Levels Purpose: Throughout this experiment we are trying to learn how to take proper pH measurements using a pH meter. We also want to see the pH of different common liquids and record the change in pH when we add acids and alkaline liquids. The last reason we are conducting this experiment is to explain and show how fresh milk spoils by the change in pH. Materials: In this experiment we used graduated cylinders and glass beakers and added variety of ingredients including 50 mL of;...

Monica Mahmoudi 02072798 Bandaruk Mon-Wed 12:45 PH Balance in the Human Body As we all know every solution is either acid or alkaline. Alkaline is often called base. These solutions can be anything from body fluids, to fruits, vegetables, seawater, beverages you name it. Acid and alkaline is measured in pH. It starts as low as zero being very acidic and works its way up to fourteen being the most basic. The pH of our stomach is 2, obviously to digest food, wine is 3.5,water is 7(neutral),...

Name: Reba Smith Date: May 27,2013 Instructor’s Name: Sylvia Nemmers Assignment: SCI103 Phase 1 Lab Report Title: Measuring pH Levels Instructions: Enter the Virtual Lab, and conduct the experiments provided before going out into the field for additional research. Please type your answers. When your lab report is complete, submit it to the Submitted Assignments area of the Virtual Classroom. Part I: Answer the following questions while in the Phase 1 lab environment. Section...

Buffers, and pH, and Diffusion oh my The pH of a solution is the measure of the concentration of charged Hydrogen ions in that given solution. A solution with a pH lower than seven is considered to be acidic. A solution with a higher pH is a base. It is very important for organisms to maintain a stable pH. Biological molecules such as proteins function only at a certain pH level and any changes in pH can result in them not functioning properly. To maintain these constant pH levels, buffer...

pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer...

pH and Microbial Growth Introduction: Each organism has an optimum environment for growth, which includes pH level. Organisms that grow at near neutral pH levels are called neutrophiles. Those that grow best in acidic pH values are called acidophiles. Others, yet, grow best in alkaline pH values and are called alkaliphiles. In this lab, each group was assigned an organism, which was then used to inoculate 6 different pH levels. Materials and Methods: Followed lab procedure 29, “pH...

Title : THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase, an enzyme found in many different tissues, catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen...

EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz, Michael Ray Dela Cruz, Karen Angelica Domalaon, Abegail Dulay, Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme, a natural catalytic agent for biochemical reactions, can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to...

Indicators and pH Measurements Author Name: Klein Partner’s Names: Peiman Instructor: Dharinee Doobur-Choytun Vancouver Community College Chemistry 0983 Section 433 Date: November 15, 2012 Due Date: November 22, 2012 Experimental Purpose Using our basic learning’s about Indicators and pH measurements, we will use our Universal Indicator and our pH Meter. By using these materials, we will experiment on how it will...

Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity, structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in, but not changed by or consumed in, a chemical reaction. The amount of energy required for a...

Melanie McGivern. Access to nursing Group 2 Effects of pH on enzyme activity Contents Front cover Aim Introduction Hypothesis Prediction Variables Materials Methods Results Discussion Conclusion Bibliography Aim The aim of the experiment is to see the enzyme amylase catalyse starch in a chemical reaction. | | Introduction Enzymes are proteins. They act as catalysts, allowing chemical reactions to take...

Winthrop University’s Successional Plot’s Soil P.H. Meredith Hamilton Biology- Westover September 30, 2011 Abstract The question of this study pertained to the amount of vegetation that was occurring on each of the succession plot’s ground floor. There are three successional plots in Winthrop University’s wooded areas, the oldest was created in 1989 the second was created in 1994 and the youngest plot was created in 1999. For the hypothesis to be tested, there were soil PH meters to...

Investigating the Properties of pH The manufacture’s claim is that Sensodyne toothpaste micro hardens tooth enamel to help protect against the effects of acid wear, helps stop the twinges of pain from sensitive teeth formulated with low abrasivity, neutral pH and gentle yet effective cleaning system. To find out if the manufactures claim on Sensodyne toothpaste is the correct level of pH. I expect the Sensodyne toothpaste to be a neutral pH level as the manufactures claim that this...

The different methods of measuring acids and bases in a solution By Starsky Intro to Biology September 26, 2011 Lab Partners: Kristen, Tania and Betty Introduction When using different methods to measure pH levels there are some tools that can be useful. Some more than others but by putting into action the different methods it may determine which tools will work best and...

The Effects of pH in Beano Introduction: The enzyme my experiment was conducted from was Beano. Beano is a dietary supplement which is used to reduce gas in the digestive system and is enzyme based. The question that my group was trying to answer is “At what pH is Beano most effective?” Our hypothesis was “If Beano solution is placed in the 6 pH buffer, than it will work more efficiently.” Methods and Materials: Two Beano tablets were grinded into a fine powder and then 10ml of water...

Abstract Scientific method is a systematic observation, measurement and experiment. It is the formulation and testing of a hypothesis. When analyzing pH it measures for the acidity or basicity of a solution. pH levels vary depending on the solution. The more acidic solutions would be juices and vinegar and cleaning materials would be basic. You can determine the purpose of solutions based on their acidity and basicity. We measure the pH of solutions on a scale with levels from 1-14. These...

Procedure: Part I: Titration with an Indicator 1. Fill the 50-milliliter buret with a 0.25 molar NaOH solution. 2. Record volume. 3. Measure out between 20 milliliters and 40 milliliters of the unknown HCl solution. 4. Record volume. 5. The amount of unknown HCl is then added to the 100-milliliter Erlenmeyer flask. 6. Add two drops of the indicator, phenolphthalein, to the acid in the flask. 7. Using the slider on the right hand side, add NaOH to the HCl in the Erlenmeyer flask (This...

The relatively close pH levels of Tap Water, Spring Water, Flavored Water, and Seltzer Water. Introduction This experiment was performed to investigate the following hypothesis: The following four different types of drinking water (spring water, seltzer water, tap water, and flavored water) we test will relatively have the same pH level. Our group decided to test different types of drinking water to see if a pH level of tap water is comparable to other types of water. Many people...

 Purpose The purpose of this laboratory is three fold: 1. Familiarize the student with the concept and meaning of pH 2. Provide the student experience in measuring pH a. pH testing paper 3. Test the student’s hypothesis as it related to the pH of common solutions Hypothesis The pH of the tested solutions will be in the order of the following according to a pH scale: 1. Lime juice 2. Orange juice 3. Soda 4. Iced Tea 5. Milk 6. Water...

The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy, speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We...

Background: Pepsinogen is an inactive protein that is the mucus lining of the stomach. When converted to pepsin, the enzyme is used to break down large or undigested protein they has been absorbed by the small intestine. The digestive power of pepsin is greatest at the acidity of normal gastric juice (pH 1.5–2.5). In the intestine the gastric acids are neutralized (pH 7), and pepsin is no longer effective. "Pepsin (biochemistry)." Encyclopedia Britannica Online. Encyclopedia Britannica, n.d....

 Design Experiment. How does pH of potatoes affect the breakdown of starch by the enzyme Salivary Amylase? Enzymes are proteins that are found inside our bodies and their roles are to break down large molecules into smaller soluble ones. Enzymes are found in different parts of our bodies and each of them breaks down a specific type of substrate at specific conditions. These conditions include pH and temperature. Our internal body temperature is around 37°C and this is the optimum...

Armadillium vulgare taxis response to different pH levels. Abstract The terrestrial isopod, Armadillium vulgare is commonly referred to as a slater or pill bug. Since transitioning from the sea to land and originally colonizing in Mediterranean regions, it has adapted throughout evolution to inhabit local microhabitats. The pill bug is bound by several parameters and also has specific requirements that need to be met for optimal biological functioning. As such, behavioural and...

 Effects of pH on the Function of Enzymes Rena Mototani Glen Rock High School Advanced Biology 2014-2015 Effects of pH on the Function of Catalase Rena Mototani Problem: How does the pH of a cell affect the function of the enzyme catalase? Introduction In this lab, we experimented the effects of pH on the function of the enzyme catalase. Catalase is an enzyme that brings about the reaction by which hydrogen peroxide is decomposed to water and oxygen (Encyclopedia...

A pH meter is an electronic device used for measuring the pH (acidity or alkalinity) of a liquid (though special probes are sometimes used to measure the pH of semi-solid substances). A typical pH meter consists of a special measuring probe (a glass electrode) connected to an electronic meter that measures and displays the pH reading. A colorimeter is a device used in colorimetry. In scientific fields the word generally refers to the device that measures the absorbance of particular...

Abstract The catalysis of enzymes within our bodies is essential for human survival and when this ability is impaired by the presence of Hydrogen ions, our cells cannot function properly. This experiment was conducted to determine if the reaction rate changes in response to a variation of acidic, neutral, and basic solutions. The experimental results indicated that the basic/high pH solution has a faster rate of reaction in the solution. Introduction Enzymes are proteins that catalyze...

Billy Kong Chem&163 Lab Report 1 Analysis of Acids, Bases, pH and Salt Purpose: Explore various methods for determining the characteristics of acid/base solution. The goal of this experiment include the investigation of various acid/base indicators, calculating the change of pH with concentration, change of pH due to the addition of salt and different between strong and weak acids/bases. Safety and Disposal: Use extreme caution when handling concentrated acids and bases. Dispose of...

Title: The Effects of Temperature and pH on Enzymatic Activity Course: Bio 120 An enzyme is a molecule that is a biological catalyst for chemical reactions in the body. Most are in the category of proteins, however a few molecules called ribozymes can also catalyze reactions. Enzymes use a specific reactant called substrates to produce products. Without the use of enzymes most of the biological reactions would occur at too slow a pace to sustain the life of a cell and the organism....

The Effect of Soil pH on the Living Conditions In Lumbricus terrestris Abstract The difference in soil pH weighs a heavy measure on the appearance of the earthworm Lumbricus terrestris in different areas of soil. A highly acidic or highly alkaline soil may be the underlying reason for the absence of this earthworm in certain areas where pH plays a large role. In this experiment, soil pH was altered with Miracid—a soil acidifier and Agricultural Limestone—a soil correctional for acidic...

Biology Experiment Effects of pH on Enzymes AIM: To investigate the effect of pH on the enzyme catalase. THEORY: The enzyme in potato is called catalase. Catalase is also produced by the body to breakdown hydrogen peroxide(H2O2). Catalase present in all body organs being especially concentrated in the liver and erythrocytes (red blood cells). The brain, heart, skeletal muscle contains only low amounts. H2O2 is common by-product of metabolic reactions. In high concentration it is toxic;...

Determining pH Using a Red Cabbage Farnoosh Mazarei Food Microbiology April 22, 2011 Purpose: To test the pH level of many common household object by use of the juice from a red cabbage. Background: pH is the measure of the acidity or basicity of a solution. The pH scale ranges from 1 to 14 with 1 through 6 being acidic and 8 through 14 being basic; 7 is considered neutral. The use of the red cabbage, or red cabbage juice is because red cabbage can be used as an acid/base...

Abstract This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was...

Investigating pH Aim: To demonstrate dissolved carbon dioxide changing the ph level of substances. Materials: * 2 beakers * 2 straws * Stop watch * Distilled water * Lime water * Litmus Paper Method: 1. Pour 100ml of Distilled water into the first beaker, and label said beaker. 2. Pour 100ml of Lime water into the second, also Label beaker. 3. Test the two liquids with litmus paper and note the ph level, before any carbon dioxide has been introduced. 4. Place a...

Effect of pH on Green Pea Germination Objective: To determine how pH affects the germination of green peas and to examine the degree of germination within each pH level. We will do this by using various buffer solutions (along with distilled water) to grow green peas. Hypothesis: We expected that the group of peas that encountered a pH of 7 to have the most and highest degree of germination. Materials: tap water buffer solutions pH 3,5, 7, 9,11...

The Effects of Varying pH on Enzyme Activity A lab was conducted to test different pH balances on an enzyme. Introduction: Enzymes are protein catalysts that speed up a chemical reaction without being consumed in the process. Enzymes are three-dimensional structures that consist of one or more polypeptide chains. The polypeptide chains form an active site (where a substrate will fit into). Enzyme molecules are folded into a very specific shape held together by the different forces of...

CAT - Measuring the pH of common household substances 1. Predict the pH value of substances & estimate the pH value by using the universal indicator. 2. Use the indicator to identify acidic, basic, and neutral substances found in any home 3. Classify household items as acids and bases 4. Collect organise and analyse results in tables and graphs (if necessary) 5. Analyse results and explain the reason the substances have the pH obtained. Aim – To use a...

THE EFFECT OF pH LEVEL ON CORROSION RATE A Long Laboratory Report Presented To; The Department of Chemical Engineering...

Background An indicator is a halochromic chemical compound that is added to a solution to determine its pH. It is a chemical detector for hydronium ions. The indicator changes colour to show the pH of the solution. Some indicates are not very precise and only tell us whether the solution is acidic or basic. When the indicator is added to the solution, they bind to hydrogen or hydroxide ions. The different electron configurations of the bound indicator cause the indicator's colour to change....

16-3 Red Cabbage Juice pH Indicator Sources: Prof. George Ewing's C100 Demonstration Notes; Prof. Carolyn Huffman's Fall '93 C100 lecture; B. Z. Shakhashiri, 1989, Chemical Demonstrations: A Handbook for Teachers of Chemistry, vol. 3, pp 162-166. Description and Concept: Red cabbage juice will change to a variety of colors when added to solutions of various pH. Red cabbage juice is a pH indicator. Materials: red cabbage* blender hot or boiling water filter paper (coffee...

 Practical 3 Title: Effect of dissolved Carbon Dioxide on the pH of Water Aim: To investigate the effect of dissolved carbon dioxide on the pH of water. Hypothesis: The dissolved carbon dioxide will cause the water to turn acid, lowering the pH when carbon dioxide dissolves in water in which it forms carbonic acid (H2CO3). Materials: Straws, distilled water, safety glasses, pH probe, computer, universal indicator, gloves, lab coats, measuring cylinder, data...

September 10th, 2013 EXPERIMENT 1 LEARNING BASIC OPERATIONS: THE EFFECT OF PH ON A FOOD PRESERVATIVE Purpose: The purpose of this experiment was to investigate a competitor’s claim that the food preservative, sodium benzoate (C6H5COONa), made by Fresh Foods International (FFI), changed into a new substance in stomach acid. Stomach acid has a pH between 1 and 3 due to the hydrochloric acid content. Based on the net equation given in the book as well as the solubility of the product our...

Effects of pH on fungal amylase activity BI 211 November 25, 2011 Introduction In recent years, the uses of microorganisms have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms. Amylase is one of the most widely used enzyme required for the preparation of fermented foods. Apart from food and starch industries, in which demand for them is increasing continuously, amylase is also used in...

 Effects of hyperventilation on CO2 and pH levels Alidrin Armandico Physiology Lab 142 Egle Ortega Aprill 25, 2014 Abstract The aim of this experiment was to analyze how pH and carbon dioxide (CO2) levels in the blood change during hyperventilation and how the renal system compensates for this condition. Hyperventilation decreases CO2, increases pH, and lowers H+ levels which then results to respiratory alkalosis. In the experiment, we used the PhysioEx 8.0 software to simulate...

 The Effect of pH on Enzymatic Reaction Rate Abstract Enzymes are affected by changes in pH. The most favorable pH value— the point where the enzyme is most active—is known as the optimum pH. This experiment was conducted to determine the effect of pH reaction rate on an enzyme, catalase, from yeast. The experimental results indicate that the catalase worked best at a neutral pH level of seven (7). Introduction An enzyme is a protein molecule that serves as a catalyst. “The basic...

Lab 1: Effect of pH on sodium benzoate, a food preservative Sheikh M Zakaria Person no. 36295651 TA: Synthia Gratia Date of Submission: 05/29/13 Abstract Sodium Benzoate is a common food preservative used in food products such as jams and jellies, soft drinks, pickles, condiments etc and in tinned products in the market. This experiment aimed to determine whether benzoic acid is formed from it’s superior soluble form sodium benzoate in stomach acid, which is simulated by HCl (pH=2). It...

Figure 1. Nutrient availability and microbial activity as affected by soil pH; the wider the band, the greater the availability or activity. (Adapted from Truog, USDA Yearbook of Agriculture 1943-1047) Rosen, C. et al. Soil pH Modification. 2004. Soil Test Interpretations and Fertilizer Management for Lawns, Turf, Gardens, and Landscape Plants.http://www.extension.umn.edu/distribution/horticulture/components/1731-03.html. University of Minnesota. (accessed January 15, 2008). Nitrogen One...

An Investigation Into The Effect Of PH On The Activity Of Potato Tissue Catalase Aim The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen. Catalase + 2H2O2 Catalase + 2H2O +O2 Catalase + Hydrogen Peroxide Catalase + Water + Oxygen Independent Variable The independent variable in this investigation is pH. Each individual enzyme has it’s own...

Experiment 1 : Design and preparation of buffers effective at different pHs Abstract The body uses natural buffers to maintain the many different pH environments in our body. This is important for optimum activity of our enzymes. When doing experiments in vitro using these enzymes it is important to mimic intracellular conditions using artificial buffer systems in order to obtain accurate results. In this experiment the buffering properties of three artificial buffer systems containing...

The Effect of Concentration, pH and Temperature on Enzyme Activity Biology For Majors October 4, 2012 Abstract We examined the reaction an enzyme has when its concentration, pH and temperature are altered. In order to do this, we added different levels of pH into different test tubes with the enzyme (sucrose), and substrate (sucrose), and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the...

Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution, then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide...

 Finding the Optimal pH of the Enzyme Peroxidase with the Aid of a Spectrophotometer ABSTRACT The peroxidase enzyme was partially purified, and the enzyme activity was calculated with the use of a spectrophotometer, demonstrating the effect of pH on peroxidase activity. The objective of this exercise was to determine the ideal pH for peroxidase activity. The data concluded that the optimum pH for peroxidase activity is 5, and the...

Discussion: Since chemical reactions that occur within a cell occur at too slow of a rate to support life, there needs to be an enzyme present to help this reaction occur quicker. Any factors that affect the enzyme activity increase the reaction to its optimal level then begin to decrease due to denaturation. In this experiment, the factor that affected the enzyme activity was pH. Since the cause of denaturation is either the removal or addition of hydrogen ions, (Freeman, et al., 2011),...

Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids, thus make up many different combinations in types, numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein, due to various reactions of amino acids to...

Purpose: The purpose of this lab was to test how objects slightly acidic or slightly basic will affect the way catalase, the enzyme tested, denatures, or breaks down. Hypothesis: If the potato is acidic, it will react with the ­H2O2 more than it will with the raw, plain potato because the acid will denature the enzyme faster. The manipulated/independent variable is the raw, plain potato while the responding/dependent variable is the other types of potatoes used in the experiment....

Acids and Bases are two groups of chemical compounds that generally have opposite characteristics. They have there own unique traits, acids taste sour, turn litmus red while bases taste bitter and turn litmus blue. The term pH relates to measuring the acidity or alkalinity of a solution, such as vinegar, or even a damp substance like soul. The neutral pH is 7, which is essentially water, with lower numbers indicating acidity and higher numbers indicating alkalinity. Hydronium ions (H3O+)...

Testing the pH of Common Household Substances Lab Background Information: The pH scale is used to determine the acidity or basicity level of liquid solutions. If a solution scores a pH level of 1-6 it is an acidic solution, 1 being the most acidic and 6 being the least acidic. If a solution rates a pH level of 7 it is a neutral solution, meaning it is neither an acidic nor a basic solution. Lastly, if a solution is 8-14 on the pH scale it is a basic solution, 8 being the least basic and 14...

Joshua McMahon IB Chemistry Matt Chase 3A 2/13/15 Finding the PH of different concentrations of orange juice. Research Question By diluting concentrated orange juice with water, can you significantly change the PH value? Introduction Across the world, it is thought and widely accepted to believe that Orange juice is the cure to many terrible diseases. However, this may not be true anymore. It has been said that Oranges are very high in vitamin C, therefore one should drink it every...

Investigating the effect of pH on the activity of the enzyme catalase. Introduction Hydrogen peroxide (H2O2) is a very pale blue liquid which appears colourless in a dilute solution, slightly more viscous than water. It is a weak acid. It has strong oxidizing properties and is therefore a powerful bleaching agent that is mostly used for bleaching paper. Catalase is a common enzyme found in all living organisms. Its functions include the conversion of Hydrogen Peroxide, a powerful...

The Effects of Heat on the pH of the Vegetable and Their Pigment PURPOSE The purpose of this experiment is to see how pH levels of certain vegetables change while being cooked in four different mediums (frying, boiling, steaming, roasting), and how the varying pH levels change the coloring of the vegetables. We will analyze how the different method of cooking/heating changes the levels of pH. BACKGROUND INFORMATION This experiment is based around the different pigments in vegetables, of...

Chapter 2: Review of Related Literature Our independent variable in this study is the materials that will be tested while our dependent variable is the color of the product. Positives: 1. According to Rhodium (2008), “The cheapest pH-meter will cost $50 and most will go for over $100. PH paper is cheaper. But you really don’t have to use them both.” 2. According to Anne (2013), “Some fruits and vegetables contain special type of chemical so it is very convenient to use.” 3. According to...

Acid-Base Titration Using a pH Meter Bradley Holloway and Jennifer Parker Period 6 April 16, 2013 Introduction: In chemistry, the ability to find molarities of acidic and basic solutions is a convenient way to convert between moles of solute and the volumes of their solutions. Through the process of titration, the molarity of these acids and bases can be found to a high level of precision. To begin titration, one solution is added to a second solution slowly until a certain...

The Effect of Starch on the Activity of Amylase with pH Variable Lab Report, Fall 2011 East Tennessee State University Department of Biological Sciences By: Shelby Brackett Date Performed: October 10, 2011 Lab Instructor: Joseph Kusi Biology 1111, Section 018 Abstract Enzymes are very important in chemical reactions. They are used to speed up the reaction taking place. They act by binding to a specific substrate and form an enzyme-substrate complex that may put stress on chemical...

Purpose Understanding how catalase activity is affected by pH is the purpose of this experiment. Introduction Enzymes play an important role in daily life because of the chemical reactions. Almost chemical reactions require the presence of enzymes to promote the metabolic process. They are known as the incredibly efficient and highly specific biological catalysts. Most enzymes are protein with the ability to enhance the rate of reaction between molecules. To catalyze a reaction, the...

KERRY ACIDS, BASES, & NEUTRALS September 20, 2010 EXPERIMENT Acids, Bases, and Neutrals Problem Are common house-hold products pHs balanced and neutral? Information pH measures how acidic or alkaline a solution is. Its concept was introduced in 1909 by biochemist Soren Sorensen. The scale ranges from 0-14. Solutions that range from 0 to 6 are considered to be Acidic. Solution that’s on the Acidic end of the scale is low in ph, high in hydrogen ion, and low in hydroxyl ions....

! Affect of pH on Porcine Pancreatic Alpha-Amylase Activity Introduction Proteins function in a variety of different ways, and one of their fundamental tasks is to act as enzymes. Enzymes are extremely important in controlling reaction speed (by initiating and regulating biological activity), cell communication, and growth. One particularly significant enzyme is amylase, which catalyzes the hydrolysis of alpha glycosidic linkages of amylose, starch components, and other oligosaccharides...

SCHOOL OF INDUSTRIAL TECHNOLOGY IMG 103/3: FOOD CHEMISTRY LABORATORY REPORT TITLE : MEASURING pH LEVELS AND WATER ACTIVITY OF FOODS NAME: MATRIC NO: GROUP: 2 DATE OF EXPERIMENTAL WORK: 13 MARCH 2013 DATE OF SUBMISSION: 20 MARCH 2013 TITLE: MEASURING pH LEVELS AND WATER ACTIVITY OF FOODS ACTIVITY 1 Introduction : This experiment was performed to investigate the pH levels of different types of food products. The pH of a solution can be determined experimentally in several...

How does the ph level of water affect its rate of evaporation? Examples: Almost not all in any normal range of pH values because changing pH has little effect on the total solids dissolved in the water. However, at very high or very low pH levels, say 12 you will see an effect from the change in boiling point (a rise). However, even this will depend on how the evaporation is being done. For a big pond or lake, higher dissolved solids (acid or base) will cause the rate of evaporation to go...

AbstractDifferent pH level may affect the growth and development of the plants. Certain key words include: pH level, germination, acidity, osmosis and diffusion. This experiment examines the effects of different pH level of vinegar on the growth of bean plants. Materials used in this experiment consist of: water (pH 6), vinegar with the pH level of 3, 4 and 5 (each one were made before experiment), beans, soil, and pots. Eight bean plants were planted, two were watered with pH 6 and the other...